Evaluation of foliar application effect of melatonin and methyl jasmonate on the phenolic and flavonoid compounds of purslane medicinal plant

Document Type : Original Article

Authors

1 Department of Agricultural Biotechnology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

2 Department of Agricultural Biotechnology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran e-mail:sharifi@uk.ac.ir Sharifisirchi@yahoo.com

3 , Department of Food Science and Technology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

4 Department of Food Science and Technology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

10.22092/mpt.2024.365969.1155

Abstract

Methyl jasmonate and melatonin, as cell regulators and biostimulants, enhance product quality and quantity by influencing growth and development processes. Given the significant effects of medicinal plants and secondary plant metabolites on human life, this study aimed to evaluate the effect of foliar application of methyl jasmonate and melatonin on phenolic and flavonoid compounds in purslane (Portulaca oleracea L.). This research was carried out in the biotechnology research greenhouse of Shahid Bahonar University of Kerman, on June 11, 2023. Foliar application was continued from August 7, that is, after vegetative growth and before the plant entered the flowering stage, with applications every two days for two weeks. The experimental treatments included distilled water (control), methyl jasmonate, melatonin, and a combination of methyl jasmonate and melatonin in two concentrations (0.1 and 0.5 mM). A completely randomized design with three replications was used for statistical analysis. The results showed that foliar application of methyl jasmonate, melatonin, and a combination of methyl jasmonate and melatonin at concentrations of 0.5 mM and 0.1 mM significantly increased the content of phenolic and flavonoid compounds in purslane (P ≤ 0.05). The highest phenol and total flavonoid content was obtained under foliar application of 0.5 mM melatonin, which was 3.28 and 0.95 times higher than the content of these compounds in the control sample, respectively. Based on the results foliar application of melatonin at a concentration of 0.5 mM is recommended due to the significant effect on increasing the total phenolic and flavonoid content .

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