نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار پژوهشی، بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش
2 بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی،
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Okra (Abelmoschus esculentus) is an important medicinal plant belonging to the Malvaceae family. In April 2023, new symptoms in greenhouse-grown okra, including twisted, curled, or wrinkled fruit capsules, growth abnormalities, shortened internodes, dwarfing, and witches’ broom, were observed in the Chenaran district of Khorasan Razavi province. To confirm the pathogenicity and identify the causal agent, the pathogen was transferred from infected okra plants to periwinkle seedlings (Catharanthus roseus) using dodder (Cuscuta campestris) under greenhouse conditions. After one month, the periwinkle plants exhibited phyllody symptoms. DNA was extracted from the midribs and petioles of five infected okra plants and three healthy plants (negative controls) for pathogen identification using the CTAB method. Direct and nested polymerase chain reaction (PCR) assays were performed using universal phytoplasma primers, P1/P7 and R16F2n/R16R2, respectively. The expected amplicons were obtained from PCR products of all five infected plants. Phylogenetic and virtual RFLP analyses using the iPhyClassifier software and in vitro RFLP with restriction enzymes revealed that the phytoplasma associated with okra's twisted pod disease closely resembled Candidatus phytoplasma trifolii from the Clover proliferation group (16SrVI-A) (accession number OR784662). This study, which provided additional data to the initial published report, not only demonstrates the expanding host range of phytoplasmas in Iran but also emphasizes the need for further research to identify other hosts and determine the precise distribution of these phytoplasmas in different parts of the country and worldwide.
کلیدواژه‌ها [English]
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