نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری تخصصی گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
2 دانش آموخته کارشناسی ارشد گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
3 دانشیار گروه علوم طبیعی و جنگلداری دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Hairy root induction by Agrobacterium rhizogenes and regeneration of modified plants from Ri-transformed roots have been reported in a wide range of plant species. Ri-transformed cultures (roots and calli) have been shown to exhibit altered characteristics compared with non-transformed cultures. Four rol genes (rolA, rolB, rolC, and rolD) within the T-DNA region of the Ri plasmid are known to be responsible for this phenomenon. In addition, the effects of A. rhizogenes on the production of secondary metabolites have been well studied in many medicinal plant species. Based on such studies, numerous papers have been published over time examining the “rol effect” on secondary metabolite accumulation in medicinal plants and discussing progress in this research area. However, investigations on the effects of individual rol genes are relatively limited and require further study. Therefore, this review discusses in detail the effects of each of the four rol genes, individually or in combination, on in vitro morphogenesis and secondary metabolite accumulation in medicinal plants. A systematic search was conducted in PubMed, Scopus, Web of Science, Google Scholar, and ScienceDirect databases using the keywords “Agrobacterium rhizogenes,” “Hairy root culture,” “rol genes (rolA, rolB, rolC, rolD),” “Secondary metabolites,” and “Medicinal plants.” The search timeframe was set from 1986 to 2024. Inclusion criteria comprised original articles related to transformation induced by A. rhizogenes, rol genes, hairy root culture, and their roles in the production of secondary metabolites in medicinal plants.
کلیدواژهها [English]
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